Fig. 3
DNA methylation regulates the transcriptional activity of BmCHT10 via its promoter. a Effects of 5-aza-dC treatment on the luciferase activities of the different truncated fragments of − 1100 to − 1 nt region of the BmCHT10 promoter in Bm12 cells. b Effects of 5-aza-dC treatment on the luciferase activities of the different truncated fragments of − 300 to − 1 nt region of the BmCHT10 promoter in Bm12 cells: − 300 to − 1 nt, − 275 to − 1 nt, − 250 to − 1 nt and − 225 to − 1 nt promoter fragments. The cells were transfected with the luciferase vector including different lengths of promoter fragments and added with 1 μL of 1 μg/μL 5-aza-dC or ddH20 (as control), respectively. c Effects of BmDnmt1 RNAi on the luciferase activity of the − 250 to − 1 nt promoter region of BmCHT10 in the Bm12 cells. dsgfp or dsBmDnmt1 was co-transfected with the vector including − 250 to − 1 nt promoter fragment for the determination of luciferase activity. Each data point is the mean ± SE of three independent assays. For the t test: p < 0.05 (*), p < 0.01(**) or p < 0.001(***)