Fig. 4

Fluorescence depolarization assay confirms the m6A specific binding of PfYTH2 protein. a Titration of varying concentrations of wild-type PfYTH2 protein titrated with cy5 labeled unmodified RNA oligos, followed by measurement of the depolarized fluorescence emission at different time points. The data points were fitted using Microsoft Excel. b Varying concentrations of wild-type PfYTH2 protein titrated with cy5 labeled m6A modified RNA oligos. c Various concentrations of PfYTH2 F98A mutant protein titrated with cy5 labeled m6A modified RNA oligos. Each concentration of protein variants was measured in triplicate and the average values were used to calculate the dissociation constant. d The calculated dissociation constant for the wild-type PfYTH2 and PfYTH2 F98A mutant protein with m6A modified and unmodified RNA oligos with standard deviation obtained from three measurements