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Fig. 6 | Epigenetics & Chromatin

Fig. 6

From: Systematic genetic and proteomic screens during gametogenesis identify H2BK34 methylation as an evolutionary conserved meiotic mark

Fig. 6

Mass spectrometry identification of post-translational modifications on core histones during sporulation. a Histones were purified during sporulation and analyzed by mass spectrometry. Technical details are presented in the Method section. b Modifications identified for each histone during sporulation. Ac, acetylation; me1/me2/me3, mono-, di- and trimethylation; ph, phosphorylation. Sites which had not been identified in yeast are highlighted in red, based on Table 3. Their MS/MS spectra are presented in Additional file 3. Phosphorylated H2B residues with ambiguous localization are indicated with a red bar. All histones were identified as N-terminally acetylated. Color coding of the residues is from SK1 background (Fig. 3)

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