Fig. 2
The lack of functional Taf14 YEATS domain does not cause any substantial growth defects in different growth conditions in yeast. Tenfold serial dilutions of taf14ΔYEATS or taf14W81A strains were spotted onto SC plates and grown at different temperatures for either 2 days (at 30 °C and 37 °C) or 5 days (at 16 °C). Cells spotted onto minimal medium (MIN) plate and cells spotted onto SC plate with galactose as a different carbon source were grown at 30 °C for 2 days. Cells spotted onto SC plate containing 0.9 M NaCl for osmotic stress were grown at 30 °C for 3 days. For DNA damage response analysis cells were spotted onto SC plates containing indicated concentrations of MMS or treatment with ionizing radiation (45–85 Gy) on SC plates was used and cells were grown at 30 °C for 2 days