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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Linker histone epitopes are hidden by in situ higher-order chromatin structure

Fig. 5

Profiles of H1 epitope exposure in HL-60/S4 cells, centered around different protein-binding sites on DNA as defined by ChIP-seq in HL-60 cells (see Methods). The different DNA-binding proteins/functions: CTCF defines chromosome loops; SMC3, subunit of cohesin; STAG1, subunit of cohesin; EGR1, transcription factor (TF); GABPA, TF; JMJD1C, histone demethylase; Pol II, RNA polymerase; REST, TF; SPI1, TF. Note that the xxChIP profiles for H1.2 and H1.5 “track” together, which always display reduced H1 epitope exposure around the center of the binding site (0). Generally, the xChIP profiles track together, sometimes in the same direction as the xxChIP profiles (EGR1, GABPA, JMJD1C, REST and SPI1); sometimes in the opposite direction (CTCF and STAG1). Interestingly, both Pol II and SMC3 indicate a divergence of the xChIP profiles around the center of the binding site

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