Fig. 4

Bull sperm tsRNA content. a Strong expression of the Glycine and Glutamine isoacceptors was observed, as well as a high proportion of reads in the 25–35 nt range b. The proportion of each category and the proportion of read length in each category are shown in (c, d), respectively. To evaluate the proportion of tsRNAs isotypes, the percentage of read counts was computed for each tsRNA category and each associated anticodon (e). Only the most expressed tsRNAs from each category are reported on the histogram. The left axis (0–40%) refers only to 5p tRHs, which are the most expressed tsRNAs. The right axis (0–10%) refers to the other tsRNAs