In vivo visualization of the i-motif DNA secondary structure in the Bombyx mori testis

Table 1 i-motif, G4, hairpin and mutant sequences used in this study

Gene ID	Wild-type and mutant sequences of the i-motif, G4 and hairpin^a	Used for
BmPOUM2-WT	TTGTTGCCCCGCCCCTCGGCCCCCTCGCGCCCCGCACTGG	BmPOUM2-WT in Fig. 1a; ssDNA, i-motif and cold probes in Fig. 2a; i-motif in Fig. 2c, Fig. 2e, Fig. 2g and Fig. 3A(a,b) and Fig. 3C; ssDNA in Fig. 2e.
BmPOUM2-Mut	TTGTTGTACTGCTACTCGGATCACTCGAGCATCGTACTGG	Mutant probes in Figs. 1b, 2a
BGIBMGA003213-WT	CCCCGCGCCCCCCGCGTCCCCCGCACCCCC	BGIBMGA003213-WT in Fig. 1c; ssDNA, i-motif and cold probes in Fig. 2b; i-motif in Figs. 2d,f, 3B(a,b), D; ssDNA in Fig. 2f.
BGIBMGA003213-Mut	CATTGCGATATCAGCGTTATCAGTATCAAT	Mutant probes in Figs. 1d, 2b
BmPOUM2-G4	CCAGTGCGGGGCGCGAGGGGGCCGAGGGGCGGGGCAACAA	BmPOUM2 G4 in Fig. 2e
BGIBMGA003213-G4	GGGGGTGCGGGGGACGCGGGGGGCGCGGGG	3213 G4 in Fig. 2f
Hairpin	CAGTGGCGGCCATCCGTTTAATATTACCGGATGGCCGCGCGAT	Hairpin in Fig. 2e, f

Hairpin was synthesized based on the tomato golden mosaic virus sequence. The i-motif, G4 and mutant sequences were synthesized based on the sequences of BmPOUM2 and BGIBMGA003213 genes
^aThe underlined based are mutated

ISSN: 1756-8935