Fig. 5
From: In vivo visualization of the i-motif DNA secondary structure in the Bombyx mori testis
Immunofluorescence detection of i-motif structures in B. mori chromosomes during the cell cycle in testis. Testes were isolated from the 4th instar larvae and treated as described in “Materials and Methods” section. a–c Staining of the i-motif (red) was observed in the interphase, prophase and metaphase of the cell cycle. d Quantification of the average number of i-motif staining signals for nuclei of testis cells. The data are represented as the mean ± SEM of three replicates. Chromosomes were counterstained with DAPI (blue)