Fig. 9

BRD4 and BRD2 promote MITF occupancy at the Tyr and Tyrp1 promoters. a Melb-a cells were differentiated and cross-linked as described in Fig. 8. ChIPs were performed with a control IgG or an antibody to MITF. MITF enrichment is shown relative to IgG enrichment and normalized to a non-specific region (IgH enhancer). The data are the average of three independent experiments. Standard error bars are shown. Statistically significant differences between (−)JQ1 and (+)JQ1 treated samples are shown (*< 0.05, **p < 0.01, ***p < 0.001). b Melb-a cells were transfected with control siRNA (siC) or a pool of siRNAs that target either BRD4 or BRD2. Cells were differentiated for 24 h then harvested for ChIPs. MITF enrichment is shown relative to IgG enrichment and normalized to a non-specific region (IgH enhancer). The data are the average of two independent experiments performed in triplicate. Standard error bars are shown. Statistically significant differences between siC-transfected cells and siBRD4 and siBRD2-transfected cells are shown (*< 0.05, **p < 0.01, ***p < 0.001)