Fig. 5

Reconstitution of the differentially methylated region by a synthetic DNA fragment in TgM. a TgM lines were generated by using the 150-kb human β-globin locus YAC bearing either the LCb or LCb118 fragments introduced 3′ to the LCR within the YAC. Filled and gray boxes indicate the “b” region (which includes Sox-Oct motifs) and 118-bp sequence, respectively. b, c DNA methylation statuses of the LCb (b) and LCb118 (c) fragments in tail somatic cells, 2-cell embryos, and sperm of YAC-TgM, that inherited the transgenes either paternally (pat.) or maternally (mat.). Regions analyzed by bisulfite sequencing were indicated by gray bars above each map