Fig. 1

Gene body methylation greatly fluctuates during honey bee development. a Schematic illustration of honey bee life cycle. Developmental stages examined in this study are in red. b Averaged CG methylation levels in the whole genome and specific annotations. Intergenic represents genomic sequences annotated as neither exonic, intronic, nor repeats. c, d Patterns of CG methylation in gene bodies in different developmental stages. Honey bee genes were aligned either at the 5′ or 3′ ends, and methylation of CG cytosines located specifically either in exons (c) or introns (d) were averaged within 100-bp intervals. The dashed lines represent points of alignment