Fig. 6

Loss of association of CAF-1 with PCNA promotes flocculation. a ASF1 and RRM3 were deleted in POL30, pol30-6 and pol30-79 strains. The cells were grown in liquid cultures, poured in Petri dishes and pictures taken with a digital camera without magnification. b cac1∆hir1∆, cac1∆rrm3∆ and cac1∆asf1∆ strains were transformed with plasmids expressing Cac1p or Cac1p with a destroyed PCNA-Interacting Peptide (cac1∆PIP). The cells were grown in liquid cultures, poured in Petri dishes and pictures taken with a digital camera without magnification. c A cac1 strain was transformed with plasmids expressing Cac1p or Cac1p with a destroyed PCNA-Interacting Peptide (cac1∆PIP) and the cells were grown in liquid cultures containing 2 and 5 mM nicotinamide (NAM). Sedimentation rates were measured and plotted as in Fig. 5