Fig. 2

TH2BS11ph modification is densely localised in the axes of the XY body. a Immunofluorescence studies of backbone TH2B and Scp3 in leptotene (1st panel), zygotene (2nd panel) and pachytene stages (3rd panel) of meiotic prophase I. b Colocalization studies of TH2BS11ph modification with synaptonemal complex protein Scp3 across leptotene (1st panel), zygotene (2nd panel) and pachytene stages (3rd panel) of meiotic prophase I. The inset in the pachytene image represents the XY body. c Colocalization studies of TH2BS11ph with γH2AX in leptotene (1st panel), zygotene (2nd panel) and pachytene spermatocytes (3rd panel). The inset in the pachytene image shows the XY body. Immunofluorescence studies of TH2BS11ph with d pATM and e ATR kinases in pachytene spermatocytes. The insets in d, e show the XY body. Data information in (a–e); All data were confirmed with at least three independent mice (C57BL6 species). Nuclei were visualised by DAPI staining, Scale bars, 10 µm. f Quantitation of colocalization percentages of TH2BS11ph with Scp3, γH2AX, pATM and ATR in the whole pachytene spermatocyte and XY body. The colocalization percentages were calculated with (with rotation) and without (without rotation) image rotation. For calculation of colocalization percentages upon image rotation, the TH2BS11ph images captured in the red channel were rotated by 90° in the anticlockwise direction in the XY plane. The number of nuclei analysed are Scp3 (n = 10), γH2AX (n = 15), pATM (n = 15) and pATR (n = 15). The data are plotted in terms of mean ± SD ***P ≤ 0.0005, **P ≤ 0.005, *P ≤ 0.05 (t-test). w rotation with rotation, wo rotation without rotation