Fig. 2

Genome-wide I-BET151 dose response of BET proteins binding profiles. a Genome-wide ChIP-seq profiles on TSS −/+ 4 Kb for BRD2, BRD3 and BRD4. All ChIP-seq profiles are RPGC (Reads Per Genomic Content) normalized followed by input subtraction. b ChIP-seq profiles obtained as in “a” for H3K27ac, H4K5ac and Pol II in MV4;11 from previously published data (52). c Genome browser visualization of two typical loci in both cell lines: KMT2E locus in K562 (left panel) and BCL2 locus in MV4;11 (right panel) showing the enrichment and the associated gradual decrease in ChIP-seq signal for all three BET proteins in DMSO and compound-treated samples. The same color codes are used for the genome browser and genome-wide profiles. The Y-scale is the same for all conditions and both cell lines for comparison purposes. Scale is indicated in the lower left corner