Fig. 2
PRDM14 dosage controls global H3K27me3 levels in migrating PGCs in both sexes. a Representative images of AP2γ-positive PGCs with upregulated and non-upregulated global levels of H3K27me3 across different Prdm14 genotypes. Scale bar: 5 µm. Intensity values for each channel along the yellow lines are plotted on the right. b Percentages of H3K27me3-upregulated and non-upregulated PGCs across Prdm14 genotypes in male and female embryos. c Percentages of H3K27me3 upregulated and non-upregulated PGCs at different developmental stages (somite number). Labels in each column indicate number of PGCs analyzed. Chi-square test was used for statistical comparisons (p < 0.001). d Representative image of a PGC and four neighboring somatic cells (same group of cells as shown in a Prdm14+/+ above). Their areas were selected to quantify the mean intensity of H3K27me3 signal. Scale bar: 5 µm. Relative intensity of H3K27me3 staining in PGCs in comparison with neighboring somatic cells across Prdm14 genotypes is plotted on the right. Medians and the 95% confidence intervals are shown as error bars. Kruskal–Wallis test was used for statistical comparison (p < 0.05)