Fig. 6

Ectopic TET2 expression reduces cell proliferation of human T-ALL. Human T-ALL cells (CCRF-CEM), engineered to ectopically express TET2 (TET2), were compared with empty vector (EV) controls. RT-qPCR analysis of a TET2 and b MYC expression. RT-qPCR data were normalized to RPL13a. c Growth curve comparing viable cell counts. d Flow cytometric cell cycle analysis based on propidium iodide (PI) staining. Cell cycle distribution (G1, S, and G2/M) displayed in percent. e Flow cytometric analysis of apoptosis based on Annexin V/PI staining. Flow cytometry profile of Annexin V staining (X axis) and PI (Y axis) is shown for representative samples. The lower right quadrant indicates the percentage of early apoptotic cells in each condition; the upper right quadrant indicates the percentage of late apoptotic cells; the upper left quadrant indicates the percentage of necrotic cells. Apoptotic cells (Annexin V-positive cells) are displayed as the percentage of gated cells. Error bars represent mean ± SEM; n = 3; two-tailed Student’s t test: *P < 0.05; **P < 0.01; ***P < 0.001