Fig. 3
TET1 is overexpressed, while TET2 is suppressed in human T-ALL cell lines and clinical specimens. Expression profiling of TET1 and TET2 in human T-ALL cell lines and clinical specimens compared to non-malignant tissue. RT-qPCR of a MYC, b TET1 and c TET2 in human T-ALL (CCRF-CEM, MOLT3, MOLT4, CCRF-HSB2, JURKAT) compared to peripheral blood mononuclear cells (PBMCs) and spleen obtained from healthy donors. RT-qPCR data were normalized to RPL13A. Error bars represent mean ± SEM; n = 3; two-tailed Student’s t test: *P < 0.05; **P < 0.01; ***P < 0.001. d TET1 and TET2 expression profiles in clinical specimens obtained though Oncomine (http://www.oncomine.org) from Haferlach et al. [33]. TET1 and TET2 mRNA levels are displayed for T-ALL (n = 174) compared to PBMCs (n = 74). Boxes indicate the interquartile range; the line within the box represents the median. Whiskers indicate the non-outlier minimum and maximum. Outliers are represented by circles. Significant P values and fold changes are indicated