Fig. 6

Defects in NuA4 or histone H4 acetylation results in accumulation of DNA damage. a Cells of the indicated genotypes were engineered to express Rad52-YFP and grown to mid-log phase at 25 °C. The percentages of nuclei with at least one Rad52-YFP focus are shown. At least 200 cells were counted for each strain in each experiment. Error bars correspond to standard errors of mean (SEM) obtained from three independent experiments. b Schematic drawing of nuclear and morphological changes during the S. pombe cell cycle. c Quantification of Rad52-YFP foci according to cell cycle stages was also conducted using the results obtained in a. For this purpose, cell length, nuclei number and position, and the presence of a division plate were analyzed as described in our previous studies [29, 32, 49, 54, 66, 72]