Fig. 2

PARP1 and RNAPII ChIP-qPCR for PARP1 target genes AKAP200 and CAPER. a Diagram of constitutive (black boxes) and alternative (gray boxes) exons of a wider region (upper) and zoomed in region (lower) for AKAP200 and CAPER genes. Green arrows depict primer locations. b, c Showing the relative occupancy of PARP1 in wild type (WT, blue line) and PARP1 knockdown (KD, red line) cells for AKAP200 and CAPER at the alternative exon relative to presiding constitutive exon. d, e Showing RNAPII occupancy (elongation form, Ser2P) for AKAP200 and CAPER. All experiments were performed in triplicate, and results are presented as mean ± SD (*p value < 0.05). Statistical significance was tested by Student’s t test method