Fig. 5
Phosphosite specific analysis. a iGPS prediction of kinases responsible for significantly regulated (p value < 0.05 based on triplicates) phosphopeptides at the indicated times after TGF-β stimulation. Top 9 kinases predicted for 5-min and 2-day regulation (compared to 0 min) were labeled in scatter plot (right). Histogram (left) showed the numbers of significantly regulated phosphosites at given stimulation times. b Scatter plots of log2 fold change of 5 min/0 min versus 60 min/0 min (up) and 1 day/0 min versus 2 days/0 min (bottom) for phosphorylation expression of kinases detected in our dataset. Kinases highlighted in yellow have been previously described with histone modifiers. c A cartoon representing signaling pathways related to histone modifications. Regulated phosphosites, which are associated with enzymatic activity, were shown with varying hues indicating the log2 fold change of relative abundance compared to 0 min. Mapk3, also named as Erk1. Rps6ka4, also named as Msk2