Fig. 4

Liver DMRs differentiating early- from advanced-stage patients with WD. WD progression-associated liver DMRs are enriched for genes involved in complement activation and embryonic development. Hypermethylated DMRs have higher methylation in advanced WD, while hypomethylated DMRs have lower methylation in advanced WD (early WD n = 3, advanced WD n = 7). a Heatmap using methylation in early versus advanced WD liver DMRs. Percent methylation for each sample relative to the mean methylation at each DMR is plotted. b Principal component analysis using methylation in early versus advanced WD liver DMRs. Ellipses show 95% confidence intervals. c Results from GREAT functional enrichment analysis of early versus advanced WD liver DMRs compared to background. Top 10 terms from gene ontology databases for hypermethylated or hypomethylated DMRs with FDR q < 0.05 are shown (dotted line indicates the significance threshold). For this and all subsequent figures: FDR; false discovery rate