Fig. 2

Kcnq1ot1 knockdown affects maternal but not paternal p57 expression. a Polymorphic fibroblasts (C57B/6 female × SD7 male) infected with the MyoD retroviral vector were transfected with Kcnq1ot1 or control siRNAs. After 24 h, cells were collected to analyze Kcnq1ot1 levels by RT-qPCR in samples depleted for Kcnq1ot1 transcript (siKcnq1ot1) compared to the control (siCTR). Values, relative to those of Tbp RNA, are the mean ± SEM of three independent experiments. Statistical significance: p value < 0.001 (***); b Allele-specific RT-qPCR analysis of p57 (M-p57: Maternal p57; P-p57: Paternal p57) expression was performed in siCTR and siKcnq1ot1 samples using allele-specific primers. Values, relative to those of Tbp RNA, are the mean ±SEM of three independent experiments. Statistical significance: p value < 0.01 (**)