Fig. 5

Detection of multimeric Insv complexes by chemical cross-linking. a Cross-linking of Thioredoxin-fused full-length Insv and 1-213-aa fragment with EGS. b–d Cross-linking of Thioredoxin-fused Insv protein fragments, as indicated, with glutaraldehyde. Protein sample concentrations in the cross-linking reaction mix ranged from 0.3 μg/mL to 1.1 μg/mL. e, f Cross-linking of controls, GST, and Thioredoxin. GST generates a dimer, while Thioredoxin remains monomeric