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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Functional dissection of the developmentally restricted BEN domain chromatin boundary factor Insensitive

Fig. 5

Detection of multimeric Insv complexes by chemical cross-linking. a Cross-linking of Thioredoxin-fused full-length Insv and 1-213-aa fragment with EGS. bd Cross-linking of Thioredoxin-fused Insv protein fragments, as indicated, with glutaraldehyde. Protein sample concentrations in the cross-linking reaction mix ranged from 0.3 μg/mL to 1.1 μg/mL. e, f Cross-linking of controls, GST, and Thioredoxin. GST generates a dimer, while Thioredoxin remains monomeric

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