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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Physiological effects of KDM5C on neural crest migration and eye formation during vertebrate development

Fig. 1

Spatiotemporal expression patterns of kdm5c during Xenopus embryogenesis. a Temporal expression patterns of kdm5c analyzed by reverse transcription-polymerase chain reaction (RT-PCR). kdm5c was strongly expressed throughout all developmental stages from the one-cell stage to the tadpole development of embryos. Ornithine decarboxylase (odc) served as a loading control. bk′ Spatial expression patterns determined by whole-mount in situ hybridization (WISH). b Xenopus embryos were collected at developmental stage 6 (cleavage stage) showing localization of kdm5c in the animal hemisphere of embryos. c kdm5c expression at the late blastula stage (st. 9) showing elevated expression levels of kdm5c in the animal pole (black arrow). d Anterior view of early neurula stage (st. 13) embryos. The expression of kdm5c in the prospective early eye field region is indicated by a black arrow. e kdm5c expression at stage 16 of developing Xenopus embryos revealing localization of kdm5c in the anterior neural tissue as indicated by a black arrow. e′ The dorsal view of neurula stage embryos exhibited kdm5c expression in the neural plate and neural plate border region and is indicated with a black arrow. f Vibratome section of stage 16 embryos showing expression of kdm5c in the neural plate and neural plate border regions. g Lateral view of early tailbud stage embryos (st. 22) indicating kdm5c expression in branchial arches with a black arrow and in the prospective eye regions with a white arrow. h Lateral view of tailbud stage (st. 32) embryos showing kdm5c expression in the brain of developing embryos. h′ Detailed view of the tailbud stage embryo in (h) showing kdm5c expression in the forebrain (black arrow), hindbrain (black arrowhead), and eye (white arrow). i Transverse section of the Xenopus embryo in (h) showing kdm5c expression in the midbrain (black arrows). i′ Detailed view of the embryo in (i) showing kdm5c expression in the retina and lens (le). j Lateral view of late tailbud stage (st. 36) embryos with kdm5c expression in the anterior regions including brain and eyes. j′ High-resolution view of the embryo in (j) revealing kdm5c expression in the retina (white arrow). k Transverse section of the embryo in (j) showing kdm5c expression in the midbrain regions of late tailbud stage embryos (black arrows). k′ High-resolution view of the embryo in (k) displaying kdm5c expression in the lens (le; dotted white circle) as well as outer nuclear layer (onl) and inner nuclear layer (inl; both marked by black arrows). Besides these regions, strong expression of kdm5c was detected in the ganglion cell layer (gcl; white arrow) of the eye and retinal pigment epithelium (rpe; white arrow)

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