Table 1 Primer set for qRT-PCR and ChIP-PCR
Gene | Primer sequences 5′ to 3′ |
|---|---|
ZEB2 | CGAGCGGCATATGGTGACA GCCACACTCTGTGCATTTGAA |
RAB25 | CTCAGCCCTGGACTCTACCAA TCCGGATGCTGTTCTGTCTCT |
CDH1 | CGGTTCCGAAGCTGCTAGTC TTGAAGCGATTGCCCCATT |
EpCAM | GCGGCTCAGAGAGACTGTG CCAAGCATTTAGACGCCAGTTT |
VIM1 | GACAATGCGTCTCTGGCACGTCTT TCCTCCGCCTCCTGCAGGTTCTT |
SIRT1 | TGTGTCATAGGTTAGGTGGTGA AGCCAATTCTTTTTGTGTTCGTG |
HPRT | TGACACTGGCAAAACAATGCA GGTCCTTTTCACCAGCAAGCT |
ChIP_RAB25_1 | ACCTCAGCCTCCCAAAGT TGAGGGCTGAGTGTGCAT |
ChIP_RAB25_2 | CCCAGCAATGCACACTCA TGGGTGGAGAGATGATGACG |
ChIP_RAB25_3 | GACACCCAACCTGTCGAACCT CGGAAGCTGAGAACAGGAAGA |
ChIP_RAB25_4 | TTTGAGAGCTGAGGGTTGAG ATCTTCCTCAGTTCCATTCCC |
ChIP_CDH1 | GGCCGGCAGGTGAAC GGGCTGGAGTCTGAACTGAC |
ChIP_EpCAM | TAGCCTCCACGTTCCTCTATCC TGCTGAGACTTCCTTTTAACCG |
ChIP_NC | CACTACGCCTGGCTAATTT TCAGGAGATCGAGACCATC |