Fig. 5
ZEB2 increased H3K9Ac deacetylation at RAB25 promoter through SIRT1 activity. a Immunoblotting of histone mark upon ZEB2 induction (+dox) in MCF7. The density of each marker was measured and represented as histograms. Expression in control was set to 1. b Histone ChIP assay analyzed at different localizations on RAB25 promoter after ZEB2 induction (+dox) in MCF7. c H3K9Ac ChIP assay performed in high (black) and low (gray) RAB25 expressing cell lines. d SIRT1 ChIP assay analyzed at different localizations on RAB25 promoter after ZEB2 induction (+dox) in MCF7. e H3K9Ac ChIP assay was performed after ZEB2 induction (+dox) with SIRT1 inhibitor (EX-527, 1 µM) (+dox/EX-527) in MCF7 and analyzed at different localizations on RAB25 promoter. For all ChIP analyses, enrichments to input were calculated, control values were set as 1 and s.d. is shown. NC negative control. P values were determined using two-way ANOVA (*p < 0.05; **p < 0.01; ***p < 0.001). Three independent experiments were performed