Fig. 3

CENP-C-, CENP-N- and CENP-T-APEX are organized differently from APEX-CENP-A but are confined inside the centromere domain. Representative images of chromatin fibers prepared from cells expressing APEX-CENP-C (a), CENP-N-APEX (c) or APEX-CENP-T (e), stained for CENP-A and biotin. Scale bar: 2.5 μm. Intensity plots for CENP-A and biotin gray values along the length of the fiber (in μm) are shown on the right. For presentation purposes, CENP-A gray values in APEX-CENP-T plot were subjected to a fivefold increase. Distribution of biotin peaks compared to APEX-CENP-A (mean with SEM), for CENP-C (b), CENP-N (d) and CENP-T (f). Dark gray: percentage of peaks co-localizing with CENP-A, light gray: percentage of peaks not co-localizing with CENP-A inside the CENP-A-bound domain, black: percentage of peaks found outside the CENP-A domain. FDR adjusted Kolmogorov–Smirnov p values are represented as follows: ns for p values > 0.05, * for p values ≤ 0.05 and ** for p values < 0.001. n = 42 fibers for CENP-A (from four experiments), 27 for CENP-C (from four experiments), 28 for CENP-N (from three experiments) and 15 for CENP-T (from two experiments)