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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: High-resolution mapping of centromeric protein association using APEX-chromatin fibers

Fig. 2

A novel approach for mapping protein binding over centromeric chromatin. a Schematic representation of the APEX-chromatin fibers workflow. b Representative images of settled U2OS cells expressing APEX-myc-CENP-A, following induction with H2O2, stained for myc, biotin and centromere marker. Insets represent threefold magnifications of the boxed regions. Scale bar: 5 μm. c Representative images of chromatin fibers prepared from cells in b, stained for CENP-A and biotin. Scale bar: 5 μm. Intensity plots for CENP-A and biotin gray values along the length of the fiber (in μm) are shown on the right. d Mean percentages of total CENP-A or biotin peaks showing co-localization with each other or not. Error bar: SEM. n = 42 fibers from four experiments

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