Fig. 2

CFP1 localisation in TSSs of housekeeping and tissue-specific genes in ERY and EBV cell types. a–c CFP1 binding specifically to TSSs in ERY (upper) and EBV (lower) cell types. Chromosomal position specified in the human hg38 genome build. Pileups are shown scaled to 1x genome coverage, with full scale 0–200x depth. a Tissue-specifically expressed loci: α-globin (chr16, left, expressed in ERY) and IRF4 (chr6, right, expressed in EBV). b Housekeeping genes: ACTB (chr7, left) and LUC7L (chr16, right). c RHBDF1 locus (chr16) with CGI promoter, but not transcribed in ERY or EBV. d Upper circles show counts of tissue-specific ERY-expressed (left), housekeeping (centre) and tissue-specific EBV lymphoid-expressed (right) genes, with total TSSs shown in parentheses. Lower pie charts show analysis of CFP1 ChIP peaks computed in accessible TSSs of ERY (red) and EBV (blue) cell types. Darker colours represent TSSs with CFP1 peaks, and dashed green lines indicate TSSs having a CGI. e CFP1 at two loci expressed in ERY: α-globin gene HBA1, expressed from a CGI TSS, compared to β-globin gene HBB, expressed from a non-CGI TSS