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Table 4 PCR cycling conditions for the generation of FISH probes

From: DNA replication and repair kinetics of Alu, LINE-1 and satellite III genomic repetitive elements

PCR cycling condition Alu MaSat
Initial denaturation 94 °C for 4 min 98 °C for 10 min
1. Denaturation 94 °C for 1 min 98 °C for 1 min
2. Annealing 57 °C/65 °C for 1 min* 56 °C for 1 min
3. Extension 72 °C for 3:30 min 72 °C for 2 min
No. of cycles steps 1.–3. 35 35
Final extension 72 °C for 10 min 72 °C for 5 min
  1. * Temperature for template PCR/temperature for labeling PCR