Table 4 PCR cycling conditions for the generation of FISH probes
From: DNA replication and repair kinetics of Alu, LINE-1 and satellite III genomic repetitive elements
PCR cycling condition | Alu | MaSat |
|---|---|---|
Initial denaturation | 94 °C for 4 min | 98 °C for 10 min |
1. Denaturation | 94 °C for 1 min | 98 °C for 1 min |
2. Annealing | 57 °C/65 °C for 1 min* | 56 °C for 1 min |
3. Extension | 72 °C for 3:30 min | 72 °C for 2 min |
No. of cycles steps 1.–3. | 35 | 35 |
Final extension | 72 °C for 10 min | 72 °C for 5 min |