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Table 3 PCR conditions for the generation of FISH probes

From: DNA replication and repair kinetics of Alu, LINE-1 and satellite III genomic repetitive elements

PCRs Alu (template) Alu (labeling) MaSat
PCR buffer* 1 × 1 × 1 ×
dATP/dGTP/dCTP 0.2 mM each 0.2 mM each 0.4 mM each
dTTP 0.2 mM 0.15 mM
dUTP-biotin 0.05 mM 0.08 mM
primer F/R 1 µM each 1 µM each 0.2 µM each
Taq polymerase 1.5 µL 1.5 µL 1 µL
DNA template 100 ng gDNA 1 µL 1:50 PCR product 100 ng gDNA
Final volume to 50 µL to 50 µL to 50 µL
  1. * 10× PCR buffer: 100 mM Tris–HCl pH 8.3, 500 mM KCl, 15 mM MgCl2