Table 3 PCR conditions for the generation of FISH probes

PCR buffer*

1 ×

1 ×

1 ×

dATP/dGTP/dCTP

0.2 mM each

0.2 mM each

0.4 mM each

dTTP

0.2 mM

0.15 mM

–

dUTP-biotin

–

0.05 mM

0.08 mM

primer F/R

1 µM each

1 µM each

0.2 µM each

Taq polymerase

1.5 µL

1.5 µL

1 µL

DNA template

100 ng gDNA

1 µL 1:50 PCR product

100 ng gDNA

Final volume

to 50 µL

to 50 µL

to 50 µL