Fig. 4

DNA repair kinetics of repetitive DNA elements assessed by FISH. a Schematics of the experiment. HeLa cells were sham-irradiated or irradiated with 2 Gy X-rays and incubated for 0.5, 3 and 24 h. γH2AX immunofluorescence and probe hybridization were performed before confocal micrographs acquisition and deconvolution. b (left) Representative confocal micrographs of HeLa cells depicting the DAPI, Alu elements and EdU as inverted gray channels, at the three time points post-IR. Merge is shown in pseudo-colors. Scale bar: 5 µm. (right) Relative change of Alu fraction in γH2AX foci. Data are normalized to the median of the 0.5 h time point. Boxes represent median, 2nd and 3rd quartile. Whiskers indicate three times the interquartile distance. Data are from three independent experiments. n combined total number of cells analyzed. sd standard deviation. c, d Represent the same as in b for LINE1 and satellite III, respectively. In d, the empty boxes represent the relative change of γH2AX intensity in segmented satellite III regions