Fig. 5

HILS1 shows unique association with LINE-1 repeats. a Visualization of selected HILS1 ChIP peaks (10 kb resolution, outlined region represents the exact peaks) generated using IGV genome browser. The y-axis unit is read per million (rpm).The x-axis represents the genomic positions in base pairs as follows: chr5:95,165,196-95,171,893(MACS_peak_32942-2:chr5); chr15:67,453,898-67,454,808 (MACS_peak_13682:chr15-1); chr10:12,608,450-12,609,577 (MACS_peak_6203:chr10-1); chr9:120,197,981-120,198,900 (MACS_peak_43102:chr9); chrX:36,777,132-36,777,862 (MACS_peak_43949:chrX); chr15:20,798,846-20,802,230 (MACS_peak_12929:chr15-2); chr1:69,879,465-69,880,705 (MACS_peak_1899:chr1); chr16:15,001,197-15,005,927 (MACS_peak_14876:chr16); chr8:39,893,407-39,894,494 (MACS_peak_39650:chr8-1); chr10:3,780,173-3,780,947 (MACS_peak_6054:chr10-2). b Quantitative ChIP PCR analysis showing the enrichment of HILS1 across specific regions represented in ChIP-seq peaks (panel A) in the chromatin fraction isolated after pull-down with anti-HILS1 antibodies. Most of the pull-down fractions showed significantly reduced enrichment in the peptide competition control group. Two negative control regions (specific regions of chr 8 and chr 18) were also included for ChIP PCR, where no peaks were present according to the data analysis and visualization. Data were represented as % of input. Results are from three independent experiments, and error bars represent standard deviation. Asterisk shows comparison with IgG control, and # shows comparison with IP+pep group. ***P ≤ 0.001; **P ≤ 0.01; *P ≤ 0.05