Fig. 2

Chromatin components of the telomeric regions. HP1, H3K9me3, and Rhi occupancies at P{EPgy2} transgenes were estimated by ChIP-qPCR using primers corresponding to 5′-P-element transgenic sequence. Primers corresponding to ORFs were used for the analysis of endogenous HeT-A, TART-A, and TAHRE. Two regions of the 42AB piRNA cluster are enriched in all studied chromatin components. rp49, metRS-m, and 60D regions are used as negative controls. Asterisks indicate statistically significant differences in Rhi enrichment relative to EY03241 (control) (*P < 0.05 to 0.01, **P < 0.01 to 0.001, ***P < 0.001, unpaired t test). The difference in the HP1 binding between transgenes is statistically insignificant