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Table 1 PCR primer sets for bisulfite sequencing analysis

From: Synthetic DNA fragments bearing ICR cis elements become differentially methylated and recapitulate genomic imprinting in transgenic mice

Regions analyzed

PCR round

5′ primers

3′ primers

I, IV

1st

lambda-MA-5S4

lambda-MA-3A2

2nd

lambda-MA-5S1

lambda-MA-3A3

II, V

1st

lambda-MA-5S5

lambda-MA-3A7

2nd

lambda-MA-5S6

lambda-MA-3A8

III, VI, VII

1st

lambda-MA-5S7

BGLB-MA-3A6

2nd

lambda-MA-5S8

BGLB-MA-3A2

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Epigenetics & Chromatin

ISSN: 1756-8935