Fig. 4

Polytenization does not takes place synchronously at all sites. a Immunolocalization of newly replicated DNA (1st pulse IdU [green], 2nd pulse CldU [red] in polytene chromosomes. To-Pro-3 was used for DNA counterstaining (blue). b Relative quantification of micronuclear gene sections in the course of macronuclear development by qPCR. c Quantification of histone variant transcripts of interest through internal normalization of each developmental time point to the histone H4 read count and normalization of each developmental time point to the read count of 15 housekeeping genes in vegetative cells. The y-axis represents the number of histone variant of interest per histone H4