Fig. 5
From: Growing oocyte-specific transcription-dependent de novo DNA methylation at the imprinted Zrsr1-DMR
The antisense transcript in the promoter region of Commd1 in adult tissues. a Strand-specific RT-PCR was performed to detect an antisense transcript to Commd1 transcription in the promoter region. The arrows represent the primers, CommUP-F1, for cDNA synthesis, and CommUP-F2 and CommUP-R2, for subsequent RT-PCR. The amplicon is 206 bp in size. CommUP-R2 is located approximately 100-bp upstream from the putative transcription start site of Commd1. b Brain (Br) and liver (Lv) RNA were analyzed. Tissues were prepared from adult F1 mice generated from crossing PA-B6 females with WT PWK males. cDNA synthesis was performed with (RTase +) or without (RTase −) reverse transcriptase. MW: molecular weight marker. c Allelic expression of the antisense transcripts was analyzed via direct sequencing of the amplified brain cDNA from the WT and PA mice shown in b. Three SNPs used to discriminate the parental alleles are shown under the electropherograms