Fig. 2
From: Growing oocyte-specific transcription-dependent de novo DNA methylation at the imprinted Zrsr1-DMR
Analysis of allelic Commd1 expression and Zrsr1-DMR methylation in PA and WT MII oocytes. a MII oocytes were prepared from Commd1PA(B6)/+(PWK) females (PA) and Commd1+(B6)/+(PWK) females (WT). RT-PCR was performed as in Fig. 1b. Amplified cDNA (250 bp) was digested with NlaIII (CATG). There are two NlaIII restriction sites in the B6 amplicon, but one of these is absent in the PWK amplicon because of a single nucleotide polymorphism (SNP) between the two strains. Adult brain RNA from each of the strains was used as the control. MW: molecular weight marker. b A 278-bp region in Zrsr1-DMR containing 15 CpG sites (B6) or 14 CpG sites (PWK) was analyzed via bisulfite sequencing. The alleles were discriminated via an SNP (C in B6 and A in PWK) located in the leftmost CpG site in the B6 sequence. Closed and open circles depict methylated and unmethylated CpGs, respectively