Fig. 7
From: H3K27me1 is essential for MMP-9-dependent H3N-terminal tail proteolysis during osteoclastogenesis
Working model. Our previous study revealed that MMP-9 catalyzes H3NT proteolysis for osteoclastogenic gene activation and that CBP/p300-mediated H3K18ac facilitates MMP-9 protease activity. Here, we depict that G9a-mediated H3K27me1 is necessary for MMP-9-dependent H3NT proteolysis in chromatin and proficient osteoclast differentiation. Without G9a-mediated H3K27me1, MMP-9 cannot localize at osteoclastogenic genes and mediate H3NT proteolysis. In the presence of G9a-mediated H3K27me1, MMP-9 gets recruited to target loci, facilitating H3NT proteolysis, leading to RANKL-induced gene transcription, and promoting osteoclast differentiation