Fig. 5

Positional organization of Sus1 in SLIK-constitutive mutants. a Heat map showing shifted 5′-end sequencing reads (tags) for Sus1 in spt71180 (left panel) and spt8Δ (right panel) at 25 °C (blue) and after 15 min at 37 °C (red), aligned by the midpoint in between the transcription start site (TSS) and transcription end site (TES), split into three subgroups: RP, SAGA and TFIID genes and sorted by gene length in each subgroup. The results of two replicates are shown. b Gene-averaged 5′-ends of shifted relative read counts (representing points of cross-linking) of Sus1-WT (left panel), Sus1 in spt71180 (middle panel) and spt8Δ (right panel) at 25 °C (blue) and after 15 min at 37 °C (red) around the transcription start site (TSS) in three gene classes: ribosomal protein (RP) genes, SAGA-dominated genes and TFIID-dominated genes, with TSS oriented to the right. Nucleosomes (based on MNase ChIP-seq) are plotted and based on values from [39]. Abrupt heat shock at 37 °C (yellow line) and 25 °C (grey fill) is shown. The resulting normalized ratios were plotted. Note that ordinate scales vary for the three gene classes due to differences in the number of genes in each class