Fig. 1

Positional organization of Sus1 before and after heat stress. a Heat map showing shifted 5′-end sequencing reads (tags) for Sus1-WT at 25 °C (blue) and after 15 min at 37 °C (red), aligned by the midpoint in between transcription start site (TSS) and transcription end site (TES). Data presented are divided into three subgroups: ribosomal protein genes (RP, n = 137), SAGA-dominated genes (SAGA, n = 471) and TFIID-dominated genes (TFIID, n = 4351) genes and sorted by gene length in each subgroup. The results of two replicates are shown. As a control, the signal of an isogenic strain bearing no-tagged Sus1 was also monitored (No-tag). b Gene-averaged 5′-ends of shifted relative read counts (representing points of cross-linking) of Sus1-WT at 25 °C (blue line) and at 37 °C (red line) around the transcription start site (TSS) in three gene classes: ribosomal protein (RP) genes, SAGA-dominated genes and TFIID-dominated genes, with TSS oriented to the right. Nucleosomes (based on MNase ChIP-seq) are plotted and based on values from [39]. Abrupt heat shock at 37 °C (yellow line) and 25 °C (grey fill) is shown. The resulting normalized ratios were plotted. Note that ordinate scales vary for the three gene classes due to differences in the number of genes in each class. c) Signal tracks, showing unshifted ChIP-exo tag 5′-end reads for Sus1-wt at 25 °C and 37 °C at RP-dependent gene RPL11 (upper panel), at SAGA-dependent gene CDC19 (middle panel) and at a TFIID-dominated gene SPB1 (lower panel) are shown