Fig. 3

Identification and affinity purification (AP) of Ibd1. a Western blot to assess whether Tetrahymena transformant cells are expressing Ibd1-FZZ. Whole-cell extract (WCE) using TCA of Ibd1-FZZ cells during vegetative growth. Ibd1 ~ 50 kDa (18 kDa FZZ + 32 kDa Ibd1). Lane 1 is untagged (−), and lanes 2, 3, 4, 5 and 6 are tagged (+, Ibd1-FZZ) Tetrahymena strains, b expression analysis of Ibd1-FZZ during vegetative growth. WCE and AP experiment extracted with a soluble-affinity buffer for untagged (−, lanes 1 and 3) and tagged (+, lanes 2 and 4) Ibd1-FZZ Tetrahymena strains. The BDR-containing protein is recognized by α-FLAG/M2 and co-purifies with Brg1 (right top panel, lane 4), c network view of Ibd1 protein–protein interactions. The edge thickness represents the averaged spectral counts for the prey. Bait proteins are shown in larger nodes which are colored according to predicted complexes as indicated