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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Individual retrotransposon integrants are differentially controlled by KZFP/KAP1-dependent histone methylation, DNA methylation and TET-mediated hydroxymethylation in naïve embryonic stem cells

Fig. 2

The KZFP/KAP1 complex protects ICRs and TEs from TET-mediated oxidation and loss of 5mC. a, b, e and f 5hmC enrichment measured by hMeDIP-qPCR in either wild-type or TET TKO cells upon Kap1 (b, f), Zfp57 (a) or Zfp932 (e) knockdown (5 days post-transduction). Error bars represent SEM, *p < 0.05, **p < 0.01, ***p < 0.001. Student’s t test. n = 4. c, d, g and h Boxplot of 5mC enrichment, measured by MeDIP-qPCR on shown loci, at indicated times after Kap1 (d, h), Zfp57 (c) or Zfp932 (g) knockdown. Data are represented as log2 of the fold change between percentages of total input for knockdown and control cells. *p < 0.05, **p < 0.01, ***p < 0.001. Student’s t test. n = 4

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Epigenetics & Chromatin

ISSN: 1756-8935