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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Individual retrotransposon integrants are differentially controlled by KZFP/KAP1-dependent histone methylation, DNA methylation and TET-mediated hydroxymethylation in naïve embryonic stem cells

Fig. 1

KAP1-dependent heterochromatin preserves DNA methylation in murine ESC. a Violin plot of the DNA methylation levels (75th percentile) measured by reduced-representation bisulfite sequencing (RRBS) on genomic regions carrying the indicated marks. b H3K9me3 ChIP-seq reads coverage in control and Kap1 knockdown mESC 5 days after transduction with shRNA-expressing lentiviral vectors. Coverage is plotted over the relative length of each H3K9me3 enriched region. Data are from two independent ChIP-seq experiments. c, d Boxplot representing 5hmC enrichment over total input on selected regions measured by hMeDIP-seq (c) or fold change between Kap1 knockdown and control sample (5 days post-transduction) on genomic loci either bound or not bound by KAP1 at baseline (d). In both cases, numbers of loci in each group are indicated on top, and statistical significance was determined by t test, *p < 0.05, ***p < 0.001. Data are averaged from two independent hMeDIP-seq experiments

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Epigenetics & Chromatin

ISSN: 1756-8935