Fig. 4
From: Stabilization of Foxp3 expression by CRISPR-dCas9-based epigenome editing in mouse primary T cells
dCas9-p300CD-mediated Foxp3 promoter acetylation and transcriptional activation. a Sequence at the Foxp3 promoter locus is shown. Each gRNA sequence is underlined and numbered #P-1 to #P-10. Transcription start sites are lettered bold red. b Foxp3 mRNA expression in each gRNA-transduced 68-41 cell stably expresses dCas9-p300CD relative to control 68-41 cells (control). Data are pooled from three independent experiments and represent the means ± SDs. c Flow cytometry analysis of Foxp3 expression in each gRNA-transduced 68-41 cell stably expresses dCas9-p300CD. Foxp3 MFI relative value to control 68-41 cells was plotted. Data are pooled from three independent experiments and represent the means ± SDs. d Flow cytometry analysis of Foxp3 expression in each gRNA-transduced 68-41 cell stably expresses dCas9-p300CD or p300 mutant. e Enrichment of acetyl histone H3 at Foxp3 TSS locus in #P-3 or #P-4 transduced 68-41 cell stably expresses dCas9-p300CD. f Flow cytometry analysis of Foxp3 expression in each clone isolated by limiting the dilution from dCas9-p300CD and #P-4 co-transduced 68-41 cells