Fig. 9
From: Internal modifications in the CENP-A nucleosome modulate centromeric dynamics
Histone acetyltransferase p300 targets CENP-A K124 for acetylation. Cells were treated for 20 h with TSA (pan HDAC inhibitor = HDACiPAN), C646 (HAT inhibitor against p300 = HATip300) or a weak pan HDAC inhibitor that targets HDAC 1, 2, 4 and 10 (HDACi1,2,4,10). Chromatin-bound histones were purified from nuclei and ran on an LT gel followed by Western analysis. Average K124ac levels were normalized against total histone H2A and plotted relative to untreated = 1. Whole cells extracts and recombinant unmodified CENP-A and CENP-A K124ac proteins were also loaded on an LT gel, followed by Western analysis to determine antibody specificity (please refer to Additional file 8: Fig. S6B)