Fig. 4

FLII controls SENP3 targeting to the DLX3 gene and regulates MLL1/2 complex assembly. a Schematic representation of the human DLX3 locus is shown with transcription start site marked as arrow and exons as red boxes. Primer pairs (DLX3.1 and DLX3.2) covering promoter or exon 3 region are indicated by color lines. b Chromatin was isolated from control HeLa cells or cells depleted from SENP3 and FLII, and ChIP assays were performed with anti-FLII antibodies. Primer pairs DLX3.1 and DLX3.2 were used to amplify DNA recovered from immunoprecipitated chromatin. Values are the average of three independent experiments performed in triplicate ± SEM (T test, **p < 0.01; ***p < 0.001, n.s. not significant). c Same as b except SENP3 antibody was used for ChIP. d HeLa cell lysate was probed with indicated antibodies to monitor the depletion of FLII and SENP3. e Same as c, except anti-Ash2L and anti-RbBP5 antibodies were used in ChIP. f Same as c, except anti-H3K4me3 antibody was used for ChIP and GAPDH primer was included in the qPCR as a control. g Same as f, except anti-ser2 (phospho) RNA Pol II antibody was used for ChIP