Fig. 5

Nucleosomal organization around the silent VSG gene arrays and DNA origins of replication. Nucleosome dyads were analyzed in a 10-kb region at the beginning or end of arrays of silent VSG genes located at the right-hand side of chromosome 9. a These dyads were analyzed within a 10-kb region at the beginning of VSG arrays (18,449 dyads), or b at the ends of these arrays of silent VSG genes (21,824 dyads). These beginning and end points are indicated by the vertical gray lines in a, b. The alignments of the regions between adjacent blocks of co-aligned VSG genes (SSR/“intergenic”) and the VSG genes and pseudogenes (VSG array) are schematically shown above each panel. c The distribution of nucleosomal dyads located in the chromosomal core regions of the T. brucei genome (434,752 dyads). The gray line represents the position of the center of the ORC1 binding site. The number of nucleosomal dyads present at each nucleotide position is expressed as a percentage of the total number of dyads in the analysis. d The distribution of nucleosomal dyads in the subtelomeric region (72,616 dyads) was aligned relative to the center of mapped ORC1 binding sites [74]. The alignment of nucleosomes with major nucleosomal dyad peaks is shown as gray ovals, with the telomere end located at the right of the panel