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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Well-positioned nucleosomes punctuate polycistronic pol II transcription units and flank silent VSG gene arrays in Trypanosoma brucei

Fig. 2

Alignment of nucleosomal dyads at pol I-, II- and III-transcribed loci in BF trypanosomes. The number of nucleosome dyads assigned to each nucleotide was summed for all aligned features. Genes were aligned relative to the TSS, SAS or PAS, indicated by the vertical black line at dyad position 0 in each panel. a The average, cumulative nucleosomal dyad distribution is shown for 5S rRNA genes and b for tRNA genes. The extent of the 119-bp 5S rRNA (a) and 74-bp tRNA (b) genes are shown by the black, rectangular arrows. The ellipses a, b indicate the span of 160-bp nucleosomes, with the ellipse centers (nucleosomal dyads) aligned to the center of the corresponding, major peaks. The nucleosomes in a are labeled I–III, and the III′ and III nucleosomes indicate identical nucleosomes in the repeating 5S array. c Alignment relative to the SAS of the first coding sequence of all PTUs. d Separate alignment of the Watson and Crick strand data relative to the SAS of the first coding sequence of all PTUs. e Alignment relative to the SAS of all coding sequences within all PTUs. f Separate alignment of the Watson and Crick strand data relative to the SAS of all coding sequence within all PTUs. g Dyad axes aligned relative to the PAS of all genes in all PTUs. h Dyad axes aligned to the Watson and Crick strand data relative to the PAS of all genes within all PTUs

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