Fig. 7

Elevated expression of repetitive DNA elements in shSmyd5 ES cells. a Fold-change expression of LINE/LTR repetitive DNA sequences in shSmyd5 ES cells relative to shLuc ES cells. p value for all <2.2e−16 (Kolmogorov–Smirnov test). b Heat map showing expression of a subset of LINE and LTR regions in shLuc and shSmyd5 ES cells. c Q-RT-PCR expression analysis of two LINE elements (p value <0.05). d Fold-change expression of LINE (left) and LTR (right) repeat subfamilies in shLuc and shSmyd5 ES cells. e De novo search for LTR retrotransposons/ERVs in the mouse genome (mm9) using LTRharvest software, and annotated using LTRdigest software. A representative full-length region with internal features is shown. f Fold-change expression of LTR internal features and LTR UTR regions between shLuc and shSmyd5 ES cells. g Browser view of RNA-Seq expression and H4K20me3, and H3K9me3 in shLuc and shSmyd5 ES cells, and SMYD5-FLAG and SMYD5-bioChIP in ES cells